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Um Método Simples de Filtração de Bancada para Isolar Pequenas Vesículas Extracelulares de Células-Tronco Mesenquimais Humanas
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Um Método Simples de Filtração de Bancada para Isolar Pequenas Vesículas Extracelulares de Células-Tronco Mesenquimais Humanas

Article DOI: 10.3791/64106-v • 09:10 min • June 23rd, 2022
June 23rd, 2022 •
1,2, 3, 4, 2, 5, 4, 1, 6,7
1Centre for Tissue Engineering and Regenerative Medicine, Universiti Kebangsaan Malaysia Medical Centre, 2My CytoHealth Sdn. Bhd., 3School of Biosciences, Faculty of Health and Medical Sciences, Taylor's University, 4School of Pharmacy, Monash University Malaysia, 5Haematology Unit, Cancer Research Centre, Institute for Medical Research (IMR), National Institutes of Health (NIH), Ministry of Health Malaysia, 6School of Pharmacy, Faculty of Health & Medical Sciences, Taylor's University, 7Centre for Drug Discovery and Molecular Pharmacology (CDDMP), Faculty of Health and Medical Sciences, Taylor's University

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Este protocolo demonstra como isolar pequenas vesículas extracelulares de células-tronco mesenquimais derivadas do cordão umbilical humano (hUC-MSC-sEVs) com uma configuração simples de bancada em escala laboratorial. A distribuição de tamanho, concentração de proteínas, marcadores de sEVs e morfologia de hUC-MSC-sEVs isolados são caracterizados por análise de rastreamento de nanopartículas, ensaio de proteína BCA, western blot e microscópio eletrônico de transmissão, respectivamente.

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