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En simpel benchtop filtreringsmetode til isolering af små ekstracellulære vesikler fra humane mesenkymale stamceller
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En simpel benchtop filtreringsmetode til isolering af små ekstracellulære vesikler fra humane mesenkymale stamceller

Article DOI: 10.3791/64106-v • 09:10 min • June 23rd, 2022
June 23rd, 2022 •
1,2, 3, 4, 2, 5, 4, 1, 6,7
1Centre for Tissue Engineering and Regenerative Medicine, Universiti Kebangsaan Malaysia Medical Centre, 2My CytoHealth Sdn. Bhd., 3School of Biosciences, Faculty of Health and Medical Sciences, Taylor's University, 4School of Pharmacy, Monash University Malaysia, 5Haematology Unit, Cancer Research Centre, Institute for Medical Research (IMR), National Institutes of Health (NIH), Ministry of Health Malaysia, 6School of Pharmacy, Faculty of Health & Medical Sciences, Taylor's University, 7Centre for Drug Discovery and Molecular Pharmacology (CDDMP), Faculty of Health and Medical Sciences, Taylor's University

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Denne protokol demonstrerer, hvordan man isolerer humane navlestrengsafledte mesenkymale stamcellers små ekstracellulære vesikler (hUC-MSC-sEV'er) med en simpel bænkindstilling i laboratorieskala. Størrelsesfordelingen, proteinkoncentrationen, sEVs markører og morfologi af isolerede hUC-MSC-sEV'er er karakteriseret ved henholdsvis nanopartikelsporingsanalyse, BCA-proteinanalyse, western blot, og transmissionselektronmikroskop.

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Tilbagetrækning nummer 184
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