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En enkel bänkfiltreringsmetod för att isolera små extracellulära vesiklar från humana mesenkymala stamceller
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En enkel bänkfiltreringsmetod för att isolera små extracellulära vesiklar från humana mesenkymala stamceller

Article DOI: 10.3791/64106-v • 09:10 min • June 23rd, 2022
June 23rd, 2022 •
1,2, 3, 4, 2, 5, 4, 1, 6,7
1Centre for Tissue Engineering and Regenerative Medicine, Universiti Kebangsaan Malaysia Medical Centre, 2My CytoHealth Sdn. Bhd., 3School of Biosciences, Faculty of Health and Medical Sciences, Taylor's University, 4School of Pharmacy, Monash University Malaysia, 5Haematology Unit, Cancer Research Centre, Institute for Medical Research (IMR), National Institutes of Health (NIH), Ministry of Health Malaysia, 6School of Pharmacy, Faculty of Health & Medical Sciences, Taylor's University, 7Centre for Drug Discovery and Molecular Pharmacology (CDDMP), Faculty of Health and Medical Sciences, Taylor's University

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Detta protokoll visar hur man isolerar humana navelsträngshärledda mesenkymala stamcellers små extracellulära vesiklar (hUC-MSC-sEVs) med en enkel bänkinställning i laboratorieskala. Storleksfördelningen, proteinkoncentrationen, sEV-markörerna och morfologin hos isolerade hUC-MSC-sEVs kännetecknas av nanopartikelspårningsanalys, BCA-proteinanalys, western blot respektive transmissionselektronmikroskop.

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