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一种从人间充质干细胞中分离小细胞外囊泡的简单台式过滤方法
JoVE Journal
Biology
Author Produced
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JoVE Journal Biology
A Simple Benchtop Filtration Method to Isolate Small Extracellular Vesicles from Human Mesenchymal Stem Cells
DOI:

09:10 min

June 23, 2022

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Chapters

  • 00:05Introduction
  • 01:09Small Extracellular Vesicle Isolation from hUC‐MSCs
  • 02:48Characterization of hUC‐MSC‐sEVs
  • 07:14Results: Nanoparticle Tracking Analysis, Biomarker Expression, and Morphology of Single hUC‐MSC‐sEVs
  • 08:39Conclusion

Summary

Automatic Translation

该协议演示了如何使用简单的实验室规模台式设置分离人脐带衍生间充质干细胞的小细胞外囊泡(hUC-MSC-sEVs)。采用纳米颗粒示踪分析、BCA蛋白测定、蛋白质印迹和透射电子显微镜分别对分离的hUC-MSC-sEV的粒径分布、蛋白浓度、sEVs标志物和形貌进行了表征。

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