Journal
/
Neuroscience
/
Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice

DOI:
10.3791/64111-v

•

08:26 min

•

August 23, 2022

•

, , , ,
1Department of Cellular Neurobiology, Graduate School of Medicine and Faculty of Medicine,The University of Tokyo

Chapters

  • 00:04Introduction
  • 01:00AAV Injection
  • 03:04Cranial Window Implantation
  • 04:36In Vivo Two‐Photon Imaging of Microglia and Neuronal Calcium Dynamics
  • 06:42Results: Microglial Dynamics and Visual Responses in a Dendritic Spine
  • 07:44Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a protocol combining adeno-associated virus injection with cranial window implantation for simultaneous imaging of microglial dynamics and neuronal activity in awake mice.

Tags

Simultaneous ImagingMicroglia DynamicsNeuronal ActivityAwake MiceAAV InjectionCranial WindowStereotaxic InstrumentMotion ArtifactPrimary Visual CortexMicroinjector

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Multi-electrode Array Recordings of Neuronal Avalanches in Organotypic Cultures

Simultaneous Electroencephalography, Real-time Measurement of Lactate Concentration and Optogenetic Manipulation of Neuronal Activity in the Rodent Cerebral Cortex

Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration

A Procedure for Implanting Organized Arrays of Microwires for Single-unit Recordings in Awake, Behaving Animals

Deep Brain Stimulation with Simultaneous fMRI in Rodents

Super-resolution Imaging of Neuronal Dense-core Vesicles

Simultaneous Long-term Recordings at Two Neuronal Processing Stages in Behaving Honeybees

Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice

Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice

Optogenetic Entrainment of Hippocampal Theta Oscillations in Behaving Mice

Read Article