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Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice
DOI:

08:26 min

August 23, 2022

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Chapters

  • 00:04Introduction
  • 01:00AAV Injection
  • 03:04Cranial Window Implantation
  • 04:36In Vivo Two‐Photon Imaging of Microglia and Neuronal Calcium Dynamics
  • 06:42Results: Microglial Dynamics and Visual Responses in a Dendritic Spine
  • 07:44Conclusion

Summary

Automatic Translation

Here, we describe a protocol combining adeno-associated virus injection with cranial window implantation for simultaneous imaging of microglial dynamics and neuronal activity in awake mice.

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