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JoVE Journal
Cancer Research

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远红荧光衰老相关β-半乳糖苷酶探针,用于通过流式细胞术鉴定和富集衰老肿瘤细胞
 
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远红荧光衰老相关β-半乳糖苷酶探针,用于通过流式细胞术鉴定和富集衰老肿瘤细胞

Article DOI: 10.3791/64176-v 14:01 min September 13th, 2022
September 13th, 2022

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Summary

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提出了一种荧光流式细胞术定量由细胞培养或小鼠肿瘤模型中化疗药物诱导的衰老癌细胞的方案。可选程序包括共免疫染色、便于大批量或时间点分析的样品固定,以及通过流式细胞术分选富集活衰老细胞。

Tags

癌症研究,第187期,衰老,癌症,化疗,流式细胞术,荧光探针,多重检测
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