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Cancer Research

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远红荧光衰老相关β-半乳糖苷酶探针，用于通过流式细胞术鉴定和富集衰老肿瘤细胞

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Cancer Research

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JoVE Journal Cancer Research

Far-Red Fluorescent Senescence-Associated β-Galactosidase Probe for Identification and Enrichment of Senescent Tumor Cells by Flow Cytometry

Please note that all translations are automatically generated. Click here for the English version.

远红荧光衰老相关β-半乳糖苷酶探针，用于通过流式细胞术鉴定和富集衰老肿瘤细胞

DOI:

10.3791/64176-v

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14:01 min

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September 13, 2022

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Amy Flor, Joanna Pagacz, DeShawn Thompson, Stephen Kron

¹Department of Molecular Genetics and Cell Biology,University of Chicago

Chapters

00:04Introduction
01:16Induction of Senescence by Chemotherapy Drugs in Cultured Cancer Cells
03:29DDAO-Galactoside Staining of Senescence-Associated Beta-Galactosidase (SA-β-Gal) in Cell or Tumor Samples
04:56Flow Cytometer Setup and Data Acquisition
06:27Flow Cytometry Data Analysis
09:01Results: Screening Tumors and Chemotherapy Agents for the Induction of Senescence
13:06Conclusion

Summary

Automatic Translation

提出了一种荧光流式细胞术定量由细胞培养或小鼠肿瘤模型中化疗药物诱导的衰老癌细胞的方案。可选程序包括共免疫染色、便于大批量或时间点分析的样品固定，以及通过流式细胞术分选富集活衰老细胞。

Tags

Keywords: Senescence Senescent Cells Flow Cytometry Fluorescent Probe β-galactosidase Tumor Cells Cell Culture Cancer Biology Cell Treatment Cell Harvesting Cell Counting Cell Staining

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