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Far-Red Fluorescerende Senescence-Associated β-Galactosidase Probe for identifisering og anrikning av Senescent tumorceller ved flowcytometri

Article DOI: 10.3791/64176-v • 14:01 min • September 13th, 2022
September 13th, 2022 •
1, 1, 1, 1
1Department of Molecular Genetics and Cell Biology, University of Chicago

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En protokoll for fluorescerende, flowcytometrisk kvantifisering av senescent kreftceller indusert av kjemoterapi narkotika i cellekultur eller i murine tumor modeller presenteres. Valgfrie prosedyrer inkluderer co-immunostaining, prøvefiksering for å lette stor batch- eller tidspunktanalyse, og anrikning av levedyktige senescentceller ved flowcytometrisk sortering.

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Kreftforskning utgave 187 Senescence kreft kjemoterapi flowcytometri fluorescerende sonde multipleks
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