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Developmental Biology
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快速有效地监测完整斑马鱼胚胎中正常和异常胞嘧啶甲基化的时空监测
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Developmental Biology
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JoVE Journal
Developmental Biology
Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos
Please note that all translations are automatically generated.
Click here for the English version.
快速有效地监测完整斑马鱼胚胎中正常和异常胞嘧啶甲基化的时空监测
DOI:
10.3791/64190-v
•
07:16 min
•
August 18, 2022
•
Sarah Avila-Barnard
,
David C. Volz
1
Department of Environmental Sciences
,
University of California, Riverside
Chapters
00:05
Introduction
01:02
Zebrafish Embryo Collection and Chemical Exposure
02:25
Dechorionation of Embryos
03:22
Immunohistochemistry Using 5-mC-Specific Antibody
04:46
Automated Imaging of Embryos Within 96-Well Plates
06:02
Results: Optimization of the Custom Module for Detection of 5-mC
06:42
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文描述了一种快速有效地对完整斑马鱼胚胎内正常和异常胞嘧啶甲基化进行时空监测的方案。
Tags
Zebrafish Embryos
5-Methylcytosine
Epigenetic Biomarker
Spatiotemporal Monitoring
Cost-efficient
Rapid
Automated Acquisition
Low Sensitivity Screening
Chemical Exposure
Environmental Stimuli
NCG Methods
Paraformaldehyde Fixation
Dechorionation
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