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Immunology and Infection

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JoVE Journal Immunology and Infection
Effektiv transfektion af in vitro transkriberet mRNA i dyrkede celler ved hjælp af peptid-poloxamin nanopartikler
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Effektiv transfektion af in vitro transkriberet mRNA i dyrkede celler ved hjælp af peptid-poloxamin nanopartikler

Article DOI: 10.3791/64288-v • 10:16 min • August 17th, 2022
August 17th, 2022 •
*1, *1, 1, 1, 1, 1, 1, 2, 1, 3, 1
1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, Third Military Medical University, 2Department of Pediatrics, Ludwig-Maximilians University of Munich, 3Department of Pharmacy, Southwest Hospital, Third Military Medical University
* These authors contributed equally

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En selvsamlet peptid-poloxamin nanopartikel (PP-sNp) er udviklet ved hjælp af en mikrofluidisk blandeanordning til at indkapsle og levere in vitro transkriberet messenger RNA. Det beskrevne mRNA/PP-sNp kunne effektivt transfektere dyrkede celler in vitro.

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Immunologi og infektion udgave 186 Genlevering in vitro transskriberet messenger RNA leveringssystemer poloxamin nanopartikel
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