JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Neuroscience
/
脑室微注射脂多糖到斑马鱼幼虫中以评估神经炎症和神经毒性
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Neuroscience
Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity
Please note that all translations are automatically generated.
Click here for the English version.
脑室微注射脂多糖到斑马鱼幼虫中以评估神经炎症和神经毒性
DOI:
10.3791/64313-v
•
07:31 min
•
August 23, 2022
•
Yulin He
,
Simon Ming Yuen Lee
2
1
State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences
,
University of Macau, Avenida da Universidade
,
2
Department of Pharmaceutical Sciences, Faculty of Health Sciences
,
University of Macau, Avenida da Universidade
Chapters
00:05
Introduction
00:39
Preparing and Mounting Zebrafish for Microinjections
02:22
Injecting the Brain Ventricle
03:08
Imaging
03:43
Determination of Gene Expression Markers
04:44
Zebrafish Locomotive Behavioral Assay
05:34
Results: Lipopolysaccharide-Induced Neuroinflammation to Assess the Levels of Proinflammatory Mediators
07:02
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该协议展示了在斑马鱼幼虫模型中将脂多糖显微注射到脑室区域,以研究由此产生的神经炎症反应和神经毒性。
Tags
Brain Ventricle
Microinjection
Lipopolysaccharide
Neuroinflammation
Neurotoxicity
Zebrafish
In Vivo Model
Anti-neuroinflammatory Drugs
Glass Capillary
Micromanipulator
Agarose
Anesthesia
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
定向脑深部结构与配送药品,病毒载体,或细胞移植Microinjections
基因传递到产后大鼠脑非心室质粒注射和电穿孔
形态分析
果蝇
幼虫外周感觉神经元树突和轴突使用遗传马赛克
手动排水的斑马鱼胚胎脑室
斑马鱼幼虫的前脑的电生理记录
在斑马鱼幼虫脊髓横断
小胶质细胞作为替代生物传感器确定纳米颗粒神经毒性
啮齿动物脑 Microinjections 的个性化针
微电极阵列技术在完整仔猪脑麻醉诱导神经毒性研究中的应用
斑马鱼脑幼虫神经元、巨噬细胞和小胶质的分离和 RNA 提取
Read Article