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Avaliação da Saúde Mitocondrial em Fibroblastos Associados ao Câncer Isolados de Esferoides de Tumores Pulmonares Multicelulares 3D
JoVE Journal
Cancer Research
Author Produced
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JoVE Journal Cancer Research
Assessment of Mitochondrial Health in Cancer-Associated Fibroblasts Isolated from 3D Multicellular Lung Tumor Spheroids
DOI:

10:26 min

October 21, 2022

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Chapters

  • 00:04Introduction
  • 01:13Preparation of Cell Suspension
  • 03:24Live-Dead Analysis of Tumor Spheroids
  • 04:05Disintegration and Cell Suspension of Tumor Spheroids
  • 05:02Cancer-Associated Fibroblast (CAF) Isolation Through Microbeads
  • 06:19Flow Cytometry-Based Analysis of ACTA2 Expression in Isolated CAFs
  • 07:28Results: Assessment of Mitochondrial Health in Cancer-Associated Fibroblasts
  • 09:30Conclusion

Summary

Automatic Translation

Esferoides tumorais 3D multicelulares foram preparados com células de adenocarcinoma pulmonar, fibroblastos e monócitos, seguidos pelo isolamento de fibroblastos associados ao câncer (CAFs) desses esferoides. CAFs isolados foram comparados com fibroblastos normais para avaliar a saúde mitocondrial estudando o potencial transmembranar mitocondrial, espécies reativas de oxigênio e atividades enzimáticas.

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