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The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion
DOI:

06:14 min

November 30, 2022

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Chapters

  • 00:05Introduction
  • 01:22Surgery and Experimental Design
  • 04:04Collection of Luminal Contents and Mucosal Tissue
  • 04:46Results: Analyzing the Mesenteric Lymph Triglyceride Concentration and Flow Rate
  • 05:13Conclusion

Summary

Automatic Translation

The present protocol describes a detailed surgical protocol for isolating flowing intestinal lymph in response to intraduodenal nutrient infusions in mice. This allows for the physiological determination of total intestinal lipid absorption and chylomicron synthesis and secretion in response to various experimental nutrients.

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