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Journal / Bioengineering / In vitro Udvælgelse af konstruerede…
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Bioengineering

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JoVE Journal Bioengineering
In vitro Udvælgelse af konstruerede transkriptionelle repressorer til målrettet epigenetisk hæmning
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In vitro Udvælgelse af konstruerede transkriptionelle repressorer til målrettet epigenetisk hæmning

Article DOI: 10.3791/64403-v • 10:44 min • May 5th, 2023
May 5th, 2023 •
1, 1, 2, 1, 1, 1,3, 2, 1,4
1San Raffaele Telethon Institute for Gene Therapy (SR-Tiget), IRCCS San Raffaele Scientific Institute, 2Center for Omics Sciences, IRCCS San Raffaele Institute, 3Institute for Biomedical Technologies, National Research Council, 4Vita-Salute San Raffaele University

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Her præsenterer vi en protokol til in vitro-udvælgelse af konstruerede transkriptionelle repressorer (ETR'er) med høj, langsigtet, stabil, on-target hæmningseffektivitet og lav genom-wide, off-target aktivitet. Denne arbejdsgang gør det muligt at reducere et indledende, komplekst repertoire af kandidat-ETR'er til en kort liste, der er egnet til yderligere evaluering i terapeutisk relevante indstillinger.

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Bioengineering udgave 195 målrettet epigenetisk hæmning geninaktivering RNA-interferens kunstige nukleaser DNA-dobbeltstrengsbrud epigenetisk redigering programmerbart DNA-bindende domæne KREB-domæne DNMT3A DNMT3L arvelige undertrykkende epigenetiske tilstande hit-and-run-natur DNA-demethylering
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