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Journal
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Biology
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在水凝胶中培养、冷冻、处理和成像整个类器官和球状体
JoVE Journal
Biology
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JoVE Journal
Biology
Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel
Please note that all translations are automatically generated.
Click here for the English version.
在水凝胶中培养、冷冻、处理和成像整个类器官和球状体
DOI:
10.3791/64563-v
•
08:07 min
•
December 23, 2022
•
Olgu Enis Tok
2
,
Gamze Demirel
3
,
Yusuf Saatci
,
Zeynep Akbulut
5
,
Ozgecan Kayalar
,
Ranan Gulhan Aktas
3,4
1
Research Institute for Health Sciences and Technologies (SABITA)
,
Istanbul Medipol University
,
2
Cellorama
,
3
Cancer and Stem Cell Research Center
,
Maltepe University
,
4
Department of Histology and Embryology, School of Medicine
,
Maltepe University
,
5
Department of Medical Biology, School of Medicine
,
Maltepe University
,
6
School of Medicine
,
Koc University
Chapters
00:04
Introduction
01:14
Culturing of the Organoids/Spheroids
02:27
Immunofluorescence Labeling of Whole-Mount Organoids/Spheroids in a Hydrogel
04:43
Freezing and Thawing of Whole-Mount Organoids/Spheroids in a Hydrogel
05:53
Results: Investigating the Whole-Mount Organoids/Spheroids in Hydrogel by Imaging
07:25
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本研究描述了在各种显微镜下培养、冷冻、解冻、处理、染色、标记和检查整个球状体和类器官的方法,同时它们在多用途装置内的水凝胶中保持完整。
Tags
Organoids
Spheroids
Hydrogel
3D Culture
Freezing
Thawing
Processing
Imaging
Microscopy
Disease Modeling
Biomarkers
HEPG2 Cells
Immunofluorescence Labeling
Fixation
Washing
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