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在水凝胶中培养、冷冻、处理和成像整个类器官和球状体
JoVE Journal
Biology
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JoVE Journal Biology
Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel
DOI:

08:07 min

December 23, 2022

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Chapters

  • 00:04Introduction
  • 01:14Culturing of the Organoids/Spheroids
  • 02:27Immunofluorescence Labeling of Whole-Mount Organoids/Spheroids in a Hydrogel
  • 04:43Freezing and Thawing of Whole-Mount Organoids/Spheroids in a Hydrogel
  • 05:53Results: Investigating the Whole-Mount Organoids/Spheroids in Hydrogel by Imaging
  • 07:25Conclusion

Summary

Automatic Translation

本研究描述了在各种显微镜下培养、冷冻、解冻、处理、染色、标记和检查整个球状体和类器官的方法,同时它们在多用途装置内的水凝胶中保持完整。

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