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1Research Institute for Health Sciences and Technologies (SABITA),Istanbul Medipol University, 2Cellorama, 3Cancer and Stem Cell Research Center,Maltepe University, 4Department of Histology and Embryology, School of Medicine,Maltepe University, 5Department of Medical Biology, School of Medicine,Maltepe University, 6School of Medicine,Koc University
Chapters
- 00:04Introduction
- 01:14Culturing of the Organoids/Spheroids
- 02:27Immunofluorescence Labeling of Whole-Mount Organoids/Spheroids in a Hydrogel
- 04:43Freezing and Thawing of Whole-Mount Organoids/Spheroids in a Hydrogel
- 05:53Results: Investigating the Whole-Mount Organoids/Spheroids in Hydrogel by Imaging
- 07:25Conclusion
Il presente studio descrive le metodologie per la coltivazione, il congelamento, lo scongelamento, la lavorazione, la colorazione, l'etichettatura e l'esame di interi sferoidi e organoidi sotto vari microscopi, mentre rimangono intatti in un idrogel all'interno di un dispositivo multiuso.
