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In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection
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Cancer Research
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JoVE Journal Cancer Research
In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection

In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection

DOI:
10.3791/64718-v

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05:36 min

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February 10, 2023

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1Lester & Sue Smith Breast Center,Baylor College of Medicine, 2Department of Medicine,Baylor College of Medicine, 3Department of Molecular & Cellular Biology,Baylor College of Medicine, Houston, TX 77030

Chapters

  • 00:04Introduction
  • 00:39Syringe and Virus Preparation
  • 01:47Animal Preparation
  • 02:27Intraductal Injection of Virus
  • 03:14Detection of Infected Cells by a Fluorescent Stereomicroscope
  • 03:58Results: Analyzing the Mammary Epithelial Cells After the Intraductal Injection
  • 04:57Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes intraductal injection of viral vectors via the teat to deliver genes of interest into the mammary epithelial cells.

Tags

In Vivo Gene DeliveryMammary Epithelial CellsMammary Intraductal InjectionMouse ModelTumor GenesisViral TransductionBromophenol BlueAnesthesiaNipple InjectionMammary Gland Dissection

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