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In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection
DOI:

05:36 min

February 10, 2023

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Chapters

  • 00:04Introduction
  • 00:39Syringe and Virus Preparation
  • 01:47Animal Preparation
  • 02:27Intraductal Injection of Virus
  • 03:14Detection of Infected Cells by a Fluorescent Stereomicroscope
  • 03:58Results: Analyzing the Mammary Epithelial Cells After the Intraductal Injection
  • 04:57Conclusion

Summary

Automatic Translation

The present protocol describes intraductal injection of viral vectors via the teat to deliver genes of interest into the mammary epithelial cells.

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