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用于研究生理或泌尿环境中驻留细胞之间的动力学和相互作用的人骨髓 3D 模型
JoVE Journal
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JoVE Journal
Biology
A Human Bone Marrow 3D Model to Investigate the Dynamics and Interactions Between Resident Cells in Physiological or Tumoral Contexts
Please note that all translations are automatically generated.
Click here for the English version.
用于研究生理或泌尿环境中驻留细胞之间的动力学和相互作用的人骨髓 3D 模型
DOI:
10.3791/64736-v
•
09:07 min
•
December 16, 2022
•
Kawtar Arizkane
2,3
,
Kevin Geistlich
2,3,4
,
Laurine Moindrot
2,3
,
Emma Risson
2,3
,
Sandrine Jeanpierre
2,3,4
,
Léa Barral
2,3
,
Anaëlle Bobard
2,3
,
Giulio Menegazzi
,
Thibault Voeltzel
2,3
,
Véronique Maguer-Satta
2,3
,
Sylvain Lefort
2,3
1
Centre de Recherche en Cancérologie de Lyon
,
2
Centre de Recherche en Cancérologie de Lyon
,
3
Université de Lyon
,
4
Centre Léon Bérard
,
5
Department of Experimental and Clinical Biomedical Sciences Mario Serio
,
Università degli Studi di Firenze
Chapters
00:04
introduction
00:45
Bead Preparation and Sterilization
01:13
3D Insert Preparation Under a Sterile Hood
02:04
Cell Line Harvesting and 3D Maintenance
03:32
Addition of Cells of Interest into the System
04:07
3D Bone Marrow-Like Experimental Results
07:50
Results: In Vitro 3D Human Bone Marrow-Like Microenvironment for Understanding the Resident Cell Interactions
08:33
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了一个易于实施的标准化的微生理系统,该系统反映了人体骨髓
体内
结构的复杂性,为精细研究广泛的正常和病理事件提供了一个相关的模型。
Tags
3D Model
Bone Marrow
Resident Cells
Physiological Context
Tumoral Context
Standardized System
Human Cell Lines
Primary Cell Populations
Bone Marrow-like Structure
Cell Harvest
In Situ Cell Localization
3D Structure Dissociation
Cellular Component
Molecular Studies
Functional Studies
BCP Beads
Polycarbonate Membrane
Cell Culture Inserts
HMEC-1 Cells
HS27A Cells
Vascular Compartment
Mesenchymal Stromal Cell Compartment
Osteoblast Differentiation
Cell Suspension
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