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纳米有限元分析:蛋白质定位的光激活定位显微镜和电子显微镜
JoVE Journal
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JoVE Journal
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Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
纳米有限元分析:蛋白质定位的光激活定位显微镜和电子显微镜
DOI:
10.3791/3995-v
•
13:13 min
•
December 03, 2012
•
Shigeki Watanabe
,
Jackson Richards
,
Gunther Hollopeter
,
Robert J. Hobson
,
Wayne M. Davis
,
Erik M. Jorgensen
1
Department of Biology, Howard Hughes Medical Institute
,
University of Utah
Chapters
00:05
Title
01:24
High Pressure Freezing
02:45
Freeze-substitution
04:27
Infiltration and Polymerization
06:15
PALM Imaging
08:37
SEM Imaging
09:29
Aligning PALM and EM Images
11:51
Representative Nano-fEM Images
12:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本地化荧光标记的蛋白质在电子显微镜下,我们描述了一种方法。荧光是第一次使用光激活定位显微技术在超薄切片本地化。然后,这些图像被对齐到相同的部分的电子显微镜照片。
Tags
Nano-fEM
Protein Localization
Photo-activated Localization Microscopy
Electron Microscopy
Fluorescence Microscopy
Immuno-electron Microscopy
Correlative Approaches
Fiducial Markers
Plastic Embedding
Diffraction Limit Of Light
Nano-resolution Fluorescence Electron Microscopy
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