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EpCAM和细胞角蛋白在正常人和小鼠血液和骨髓中表达上皮细胞的证据
JoVE Journal
Biology
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JoVE Journal Biology
Evidence for EpCAM and Cytokeratin Expressing Epithelial Cells in Normal Human and Murine Blood and Bone Marrow
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EpCAM和细胞角蛋白在正常人和小鼠血液和骨髓中表达上皮细胞的证据

DOI:
10.3791/65118-v

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08:02 min

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April 21, 2023

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, ,
1The Hormel Institute,University of Minnesota

Chapters

  • 00:04Introduction
  • 00:58Harvesting Bone Marrow from Mice
  • 03:54Red Blood Cell Lysis of Bone Marrow
  • 04:38Flow Cytometry
  • 06:18Results: Flow Cytometric Analysis of EpCAM+ Cells in Murine and Human Bone Marrow and Blood
  • 07:26Conclusion

Summary

Automatic Translation

Automatic Translation

本文提出了一种可重复的方法，使用流式细胞术和免疫荧光显微镜对正常人和小鼠血液和骨髓中上皮细胞的存在有了新的发现。Krt1-14;mTmG转基因小鼠用作 体内 方法以证实这些发现。

Tags

Keywords: EpCAMCytokeratinEpithelial CellsBloodBone MarrowFlow CytometryCirculating Tumor CellsDevelopmental BiologyStem Cell BiologyRegenerative TherapiesWound HealingMouseBone HarvestBone Marrow Extraction

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