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基因枪子弹的制备和神经元的基因枪转染切片文化
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture
Please note that all translations are automatically generated.
Click here for the English version.
基因枪子弹的制备和神经元的基因枪转染切片文化
DOI:
10.3791/675-v
•
17:42 min
•
February 13, 2008
•
Georgia Woods
,
Karen Zito
1
Center for Neuroscience
,
University of California, Davis
Chapters
00:02
Title
01:02
Introduction
01:51
Preparing DNA-Coated Gold Beads
05:36
Coating Plastic Tubing
08:51
Cutting the Plastic Tubing
10:18
Shooting Tissue Slices
14:20
Results
15:53
Troubleshooting
17:04
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一个准备DNA涂金子弹的方法,并展示了使用这种子弹biolistically转染培养的海马神经元。
Tags
Gene Gun Bullets
Biolistic Transfection
Neurons
Slice Culture
DNA Coated Particles
Rat Hippocampal Slices
Gene Gun Transfection
Fluorescent Labeling
Gold Particles
Plastic Tubing
Plastic Cartridges
Bio-Rad Helios Gene Gun
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