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遺伝子銃の箇条書きとスライス培養における神経細胞の微粒子銃トランスフェクションの準備
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture
Please note that all translations are automatically generated.
Click here for the English version.
遺伝子銃の箇条書きとスライス培養における神経細胞の微粒子銃トランスフェクションの準備
DOI:
10.3791/675-v
•
17:42 min
•
February 13, 2008
•
Georgia Woods
,
Karen Zito
1
Center for Neuroscience
,
University of California, Davis
Chapters
00:02
Title
01:02
Introduction
01:51
Preparing DNA-Coated Gold Beads
05:36
Coating Plastic Tubing
08:51
Cutting the Plastic Tubing
10:18
Shooting Tissue Slices
14:20
Results
15:53
Troubleshooting
17:04
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我々は、DNAコートした金の弾丸の製造方法を説明し、biolistically培養海馬スライスの神経細胞をトランスフェクトするためにそのような弾丸の使用方法を示しています。
Tags
Gene Gun Bullets
Biolistic Transfection
Neurons
Slice Culture
DNA Coated Particles
Rat Hippocampal Slices
Gene Gun Transfection
Fluorescent Labeling
Gold Particles
Plastic Tubing
Plastic Cartridges
Bio-Rad Helios Gene Gun
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