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嗜中性粒细胞的分离协议
JoVE Journal
Biology
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JoVE Journal Biology
Neutrophil Isolation Protocol
DOI:

07:12 min

July 23, 2008

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Chapters

  • 00:00Introduction
  • 00:08Equipment
  • 00:24Materials
  • 01:24Preparation of HBSS/HSA
  • 02:06Blood sample
  • 02:38Stages of neutrophil isolation
  • 02:53First separation: acquire neutrophils
  • 04:56Second separation: refine neutrophil layer
  • 05:39Third separation: lysing the red blood cells
  • 06:27Fourth separation: resuspending the neutrophils

Summary

Automatic Translation

嗜中性粒细胞之间的第一个细胞到达炎症免疫反应的工地上,和其职能和机制,已被广泛地在体外研究。我们展示一个标准密度梯度分离法,隔离人类使用市售的分离介质从整个血液中性粒细胞。

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