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嗜中性粒细胞的分离协议
JoVE Journal
Biology
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Biology
Neutrophil Isolation Protocol
Please note that all translations are automatically generated.
Click here for the English version.
嗜中性粒细胞的分离协议
DOI:
10.3791/745-v
•
07:12 min
•
July 23, 2008
•
Hana Oh
,
Brian Siano
,
Scott Diamond
1
Institute for Medicine and Engineering
,
University of Pennsylvania
Chapters
00:00
Introduction
00:08
Equipment
00:24
Materials
01:24
Preparation of HBSS/HSA
02:06
Blood sample
02:38
Stages of neutrophil isolation
02:53
First separation: acquire neutrophils
04:56
Second separation: refine neutrophil layer
05:39
Third separation: lysing the red blood cells
06:27
Fourth separation: resuspending the neutrophils
Summary
Automatic Translation
English (Original)
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Automatic Translation
嗜中性粒细胞之间的第一个细胞到达炎症免疫反应的工地上,和其职能和机制,已被广泛地在体外研究。我们展示一个标准密度梯度分离法,隔离人类使用市售的分离介质从整个血液中性粒细胞。
Tags
Neutrophil Isolation
PMN Isolation
Leukocytes
Inflammatory Immune Response
Neutrophil Functions
Clean Method
Fast Method
Reliable Method
Density Gradient Separation
Sodium Metrizoate
Dextran 500
Layering Whole Blood
Centrifugation
Lysis Of Erythrocytes
Cell Counting
Viability Assessment
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