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人类冠状病毒的滴定使用免疫过氧化物酶含量
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Titration of Human Coronaviruses Using an Immunoperoxidase Assay
DOI:

12:00 min

April 28, 2008

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Chapters

  • 00:11Titering Coronaviruses
  • 00:54Introduction
  • 01:36Purification of Virus from Tissue
  • 03:43Purification of Virus from Cultured Cells
  • 05:31Preparation of suseptible cells
  • 08:20Virus Detection with the Immunoperoxidase Assay
  • 11:26Conclusion

Summary

Automatic Translation

在这段视频中,我们展示了另一种方法为使用酶抗原检测技术,被称为免疫过氧化物酶检测病毒的检测和滴定。在这里,我们将显示你如何收集病毒样本,准备测试的细胞,并最终免疫过氧化物酶检测采用连续稀释,以确定病毒滴度。

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