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Biology
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小鼠卵黄囊和AGM早期造血干细胞的分离
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Biology
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JoVE Journal
Biology
Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM
Please note that all translations are automatically generated.
Click here for the English version.
小鼠卵黄囊和AGM早期造血干细胞的分离
DOI:
10.3791/789-v
•
10:38 min
•
June 27, 2008
•
Kelly Morgan
,
Michael Kharas
,
Elaine Dzierzak
,
D. Gary Gilliland
3
1
Department of Hematology and Oncology
,
Brigham and Women’s Hospital and Harvard Medical School
,
2
Department of Cell Biology and Genetics
,
Erasmus University Medical Center
,
3
Department of Medicine, Howard Hughes Medical Institute
,
Brigham and Women’s Hospital and Harvard Medical School
Chapters
00:00
Title
00:51
Introduction
02:04
Dissection and Isolation of Early Hematopoietic Stem Cells
07:19
Dissociation and FACS Analysis
09:57
Conclusion
Summary
Automatic Translation
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Automatic Translation
该视频显示如何微观剖析，从胚胎的卵黄囊和主动脉 - 性腺 - 中肾区，使用流式细胞仪检测造血干细胞进行排序。
Tags
Isolation
Early Hematopoietic Stem Cells
Murine Yolk Sac
AGM
Mouse Embryo
Blood System
Fetal Liver
Bone Marrow
Microdissection
Flow Cytometric Sorting
Pure Population
Stem Cell Pool
Functional Differences
Genetic Models
Video Demonstration
FACS Plot
Functional Assays
Colony Assays
Bone Marrow Transplants
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