8,419 Views
•
11:40 min
•
October 20, 2014
DOI:
Summary
Automatically generated
Manipulating temporal gene expression in differentiating embryonic stem cells (ESCs) can be achieved using inducible gene systems. However, generation of these cell lines is costly and time consuming. This protocol achieves rapid expression of a transgene in differentiating ES-derived cells and subsequent analysis of downstream hematopoietic differentiation.
Related Videos
20:38
Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)
Related Videos
23596 Views
22:06
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
Related Videos
13271 Views
11:56
Derivation of Human Embryonic Stem Cells by Immunosurgery
Related Videos
33746 Views
10:38
Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM
Related Videos
14641 Views
09:11
Embryonic Stem Cell-Derived Endothelial Cells for Treatment of Hindlimb Ischemia
Related Videos
11680 Views
13:11
Video Bioinformatics Analysis of Human Embryonic Stem Cell Colony Growth
Related Videos
12691 Views
08:11
Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors
Related Videos
15280 Views
06:54
Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies
Related Videos
13837 Views
14:22
Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells
Related Videos
13044 Views
12:03
Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
Related Videos
18576 Views
Read Article
Cite this Article
Bikorimana, E., Lapid, D., Choi, H., Dahl, R. Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation. J. Vis. Exp. (92), e52022, doi:10.3791/52022 (2014).
Copy