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Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
JoVE Journal
Biology
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JoVE Journal Biology
Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
DOI:

07:47 min

August 14, 2009

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Chapters

  • 00:05Title
  • 01:02Introduction
  • 01:34Preparation of the CBB Staining Solution
  • 02:38SDS-PAGE
  • 04:26Staining of the Gel
  • 06:38Representative Results / Outcome
  • 07:19Conclusion

Summary

Automatic Translation

A short protocol for protein staining with Coomassie Brilliant Blue (CBB) G-250 in polyacrylamide gels is described without using organic solvents or acetic acid as in the classical staining procedures with CBB.

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