10:16Observing Viral Fusion Kinetics Under the Microscope
13:10Viral Hemifusion and Pore Formation Data: Microscope Footage, Fluorescence Intensity Trajectories, and Lag Times for Viral Hemifusion and Pore Formation
We present an in vitro, two-color fluorescence assay to visualize the fusion of single virus particles with a fluid target bilayer. By labeling viral particles with fluorophores that differentially stain the viral membrane and its interior, we are able to monitor the kinetics of hemifusion and pore formation.