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Imaging of Estrogen Receptor-α in Rat Pial Arterioles using a Digital Immunofluorescent Microscope
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Biology
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JoVE Journal Biology
Imaging of Estrogen Receptor-α in Rat Pial Arterioles using a Digital Immunofluorescent Microscope

Imaging of Estrogen Receptor-α in Rat Pial Arterioles using a Digital Immunofluorescent Microscope

DOI:
10.3791/3203-v

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07:42 min

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November 29, 2011

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, , ,
1Graduate School of Nursing,Uniformed Services University of the Health Sciences

Chapters

  • 00:05Title
  • 01:42Isolation and Preparation of Pial Arteries
  • 03:24ER-α Immunofluorescent Staining: Day 1
  • 04:36ER-α Immunofluorescent Staining: Day 2
  • 05:30Digital Fluorescence Imaging
  • 06:13Immunofluorescent ER-α Staining with Negative Controls
  • 07:03Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this article is to demonstrate a method to optimize immunofluorescent detection of estrogen receptor-α (ERα) in rat pial arterial slices using a digital immunofluorescent microscope.

Tags

Estrogen Receptor-alphaRat Pial ArteriolesDigital Immunofluorescent MicroscopeEstrogen ReceptorsFluorescent StainingConfocal Laser Scanning MicroscopyER-alphaSmooth MuscleEndothelial CellsRat Basilar ArteriesImmunohistochemical TechniquesCross-sectional SlicesPial ArteriesCryostatVessel Planes

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