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Microdialysis of Ethanol During Operant Ethanol Self-administration and Ethanol Determination by Gas Chromatography
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Chapters
- 00:05Title
- 01:39Operant Training and Pre-microdialysis Tethering and Probe Insertion
- 03:54Collection of Samples during Self-administration Sessions with Appetitive and Consummatory Phases Separated
- 06:16Preparation and Analysis of Ethanol in the Dialysis Sample
- 08:22Representative Ethanol Data
- 09:30Conclusion
A method to determine the time course of ethanol concentration in the brains of rats during operant ethanol self-administration is described. Gas chromatography with flame ionization detection is used to quantify ethanol in the dialysate samples, because it has the sensitivity required for the small volumes that are generated.
Tags
MicrodialysisEthanolOperant Self-administrationGas ChromatographyPharmacological EffectsDrugs Of AbuseOrally Administered DrugsIntravenously Administered DrugsPharmacokineticsBrain Dialysate Ethanol ConcentrationsMicrodialysis-behavioral ExperimentsStereotaxic SurgerySample CollectionDialysate Ethanol AnalysisGas Chromatography With Flame-ionization Detection
