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An in vivo Assay to Test Blood Vessel Permeability
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An in vivo Assay to Test Blood Vessel Permeability

An in vivo Assay to Test Blood Vessel Permeability

DOI:
10.3791/50062-v

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07:03 min

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March 16, 2013

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,
1Fox Chase Cancer Center

Chapters

  • 00:03Title
  • 01:04Intravenous injection of Evans Blue in the mouse lateral tail vein
  • 03:35Organ collection and extraction of Evans Blue from the organs
  • 05:15Quantification of Evans Blue extravasated in interstitial tissue
  • 06:08Conclusion

Summary

Automatic Translation

Automatic Translation

We are presenting an in vivo assay to test blood vessel permeability. This assay is based on intravenous injection of a dye and subsequent visualization of its diffusion into interstitial spaces.

Tags

Evans BlueIn Vivo AssayBlood Vessel PermeabilityEndotheliumVascular PermeabilityAlbuminExtravasationQuantitative MeasurementFormamide ExtractionOptical Density

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