/
/
Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:30Virus Inoculation of Nicotiana benthamiana and Fluorescein-5-maleimide-labeling of Virus-Like Particles
- 03:33Cross-linking of Cys 2-VLPs and F Peptide
- 04:48Representative VLP-Peptide Crosslinking Results
- 08:01Conclusion
A method to analyze the solvent accessibility of the thiol group of cysteine residues of Maize rayado fino virus (MRFV)-virus-like particles (VLPs) followed by a peptide cross-linking reaction is described. The method takes advantage of the availability of several chemical groups on the surface of the VLPs that can be targets for specific reactions.
