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Molecular Imaging to Target Transplanted Muscle Progenitor Cells
JoVE Journal
Medicine
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JoVE Journal Medicine
Molecular Imaging to Target Transplanted Muscle Progenitor Cells
DOI:

09:24 min

March 27, 2013

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Chapters

  • 00:05Title
  • 02:17Culture and Transfection of C2C12 Myoblasts
  • 04:26Preparation of Myoblasts for Transplant
  • 05:16Cell Implantation
  • 06:49Injection of the Fluorescent Substrate
  • 07:13Bioluminescence Imaging to Image Luciferase-Expressing Myoblast Progenitor Cells
  • 07:37Results: Molecular Imaging to Target Transplanted Muscle Progenitor Cells
  • 08:42Conclusion

Summary

Automatic Translation

A non-invasive means to evaluate the success of myoblast transplantation is described. The method takes advantage of a unified fusion reporter gene composed of genes whose expression can be imaged with different imaging modalities. Here, we make use of a fluc reporter gene sequence to target cells via bioluminescence imaging.

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