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Chapters
- 00:05Title
- 02:17Culture and Transfection of C2C12 Myoblasts
- 04:26Preparation of Myoblasts for Transplant
- 05:16Cell Implantation
- 06:49Injection of the Fluorescent Substrate
- 07:13Bioluminescence Imaging to Image Luciferase-Expressing Myoblast Progenitor Cells
- 07:37Results: Molecular Imaging to Target Transplanted Muscle Progenitor Cells
- 08:42Conclusion
A non-invasive means to evaluate the success of myoblast transplantation is described. The method takes advantage of a unified fusion reporter gene composed of genes whose expression can be imaged with different imaging modalities. Here, we make use of a fluc reporter gene sequence to target cells via bioluminescence imaging.
