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Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA
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Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

DOI:
10.3791/50354-v

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10:40 min

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September 10, 2013

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, , , , ,
1Biochemistry and Molecular Biology,Colorado State University

Chapters

  • 00:05Title
  • 01:37Assembly of Recombinant Core Histones into Octamers
  • 03:14Purification of Histone Octamers by Size Exclusion Chromatography |
  • 05:32Sedimentation Velocity Analysis of Reconstituted Nucleosomal Arrays
  • 07:50Results: Nucleosomal Arrays from Recombinant Xenopus Core Histones
  • 09:56Conclusion

Summary

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A method is presented for the reconstitution of model nucleosomal arrays from recombinant core histones and tandemly repeated nucleosome positioning DNA. We also describe how sedimentation velocity experiments in the analytical ultracentrifuge, and atomic force microscopy (AFM) are used to monitor the extent of nucleosomal array saturation after reconstitution.

Tags

Nucleosomal ArrayRecombinant Core HistonesNucleosome Positioning DNASedimentation VelocityAnalytical UltracentrifugeAtomic Force MicroscopyChromatin StructureChromatin Function

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