Journal
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Immunology and Infection
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Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

DOI:
10.3791/50389-v

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06:27 min

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May 06, 2013

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,
1Department of Microbiology & Immunology,Pennsylvania State University College of Medicine

Chapters

  • 00:05Title
  • 01:11Isolation of T Cells from Spleen and Lymph Nodes of BDC2.5 Mice
  • 02:52Fluorescence-activated Cell Sorting of Diabetogenic CD4+ T Cells from BDC2.5 Mice
  • 04:28Adoptive Transfer of Diabetogenic CD4+ T Cells from BDC2.5 Mice
  • 05:12Monitoring Recipient Mice for Hyperglycemia and T1D
  • 05:36Results: Adoptive T-cell Transfer
  • 06:10Conclusion

Summary

Automatic Translation

Automatic Translation

We provide a reproducible method to induce type 1 diabetes (T1D) in mice within two weeks by the adoptive transfer of islet antigen-specific, primary CD4+ T cells.

Tags

Accelerated Type 1 DiabetesAdoptive TransferDiabetogenic CD4+ T CellsNOD MouseAutoimmune DiabetesT Cell Receptor BDC2.5NOD.SCID RecipientT1D PathogenesisTherapeutic Interventions

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