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Chemistry

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Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins
 

Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins

Article DOI: 10.3791/50875-v 13:59 min December 12th, 2013
December 12th, 2013

Chapters

Summary

Two methods for assigning the α- and ε-dimethylamine nuclear magnetic resonance signals of a reductively 13C-methylated N-terminal lysine are described. One method utilizes the pH-induced selectivity of the reductive methylation reaction, and the other uses aminopeptidase to selectively remove the N-terminal lysine.

Tags

NMR Resonances 13C-Dimethyl N-terminal Amine Reductively Methylated Proteins NMR Spectroscopy Magnetic Isotopes Sequential Resonance Assignment Reductive 13C-methylation Chemical Reaction Primary Amino Groups 13C-dimethylamino Groups Structure And Function Of Proteins Metabolic Labeling Sparse Isotopic Labels Mass Spectrometry (MS) Protein 13C-dimethylamine NMR Signals Assignment Method Lysine Residue
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