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Chapters
- 00:05Title
- 03:53Protein Production and Purification
- 05:24Preparation of Proteoliposomes
- 08:35Assessment of the Reconstitution Efficacy on Sucrose Gradient
- 09:37Fluorescence Staining
- 10:28Monitoring Pyranine Fluorescence as a Function of Time While a Proton Gradient is Generated
- 12:40Conclusion
We present a protocol for the in vitro investigation of efflux pumps from Pseudomonas aeruginosa. This protocol allows for the generation of a robust, reversible, and tunable proton gradient within the liposome membrane and hence should be adaptable to any membrane protein energized by the protomotive force.
