Beobachtung und Quantifizierung der Fibroblasten-vermittelten Fibrin Gel Verdichtungs


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De Jesús, A. M., Sander, E. A. Observing and Quantifying Fibroblast-mediated Fibrin Gel Compaction. J. Vis. Exp. (83), e50918, doi:10.3791/50918 (2014).

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Name Company Catalog Number Comments
Sigma-Aldrich F8630
Sigma-Aldrich T4648
Gibco 11965-092
Gibco 15140-122
Sigma-Aldrich A2942
Sigma-Aldrich H0887
Sigma-Aldrich 223506
Gibco 25200-056
Invitrogen 3000
Lonza DE14-701F
Molecular Probes F8858
GIBCO A10483-01
GIBCO 11430-030
Fisher-Scientific SS264-1
Sigma-Aldrich A3428-25MG
Biotense Bioreactor ADMET
Ti-Eclipe Microscope Nikon
# 0 35 mm Glass Bottom Petri Dish MatTek P35G-0-20-C
# 0 35 mm Glass Top Petri Dish MatTek P35GTOP-0-20-C
Plastic Luer fittings, PVC tubing with Luer ends Cole-Parmer 30600-65



  1. Grinnell, F., Petroll WM, Cell motility and mechanics in three-dimensional collagen matrices. Annu. Rev. Cell. Dev. Biol. 26, 335-361 (2001).
  2. Sander, E. A., Barocas, V. H. Biomimetic collagen tissues: collagenous tissue engineering and other applications. In: Collagen: Structure and Mechanics. Springer. New York. (2008).
  3. Pedersen JA,, Swartz MA, Mechanobiology in the third dimension. Ann. Biomed. Eng. 33, (11), 1469-1490 (2005).
  4. Bell, E., Ivarsson, B., Merrill, C. Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. Proc. Natl. Acad. Sci. U.S.A. 76, (3), 1274 (1979).
  5. Guidry, C., Grinnell, F. Studies on the mechanism of hydrated collagen gel reorganization by human skin fibroblasts. J. Cell. Sci. 79, (1), 67-81 (1985).
  6. Fluck, J., Querfeld, C., Cremer, A., Niland, S., Krieg, T., Sollberg, S. Normal human primary fibroblasts undergo apoptosis in three-dimensional contractile collagen gels. J. Invest. Dermatol. 110, (2), 153-157 (1998).
  7. Rosenfeldt, H., Grinnell, F. Fibroblast quiescence and the disruption of ERK signaling in mechanically unloaded collagen matrices. J. Biol. Chem. 275, (5), 3088-3092 (2000).
  8. Nakagawa, S., Pawelek, P., Grinnell, F. Long-term culture of fibroblasts in contracted collagen gels: Effects on cell growth and biosynthetic activity. J. Invest. Dermatol. 93, (6), 792-798 (1989).
  9. Harris, A. K., Stopak, D., Wild, P. Fibroblast traction as a mechanism for collagen morphogenesis. Nature. 290, 249-251 (1981).
  10. Stopak, D., Harris AK, Connective tissue morphogenesis by fibroblast traction: I. tissue culture observations. Dev. Biol. 90, (2), 383-398 (1982).
  11. Sawhney, R. K., Howard, J. Slow local movements of collagen fibers by fibroblasts drive the rapid global self-organization of collagen gels. J. Cell. Biol. 157, (6), 1083-1092 (2002).
  12. Provenzano, P. P., Inman, D. R., Eliceiri, K. W., Trier, S. M., Keely, P. J. Contact guidance mediated three-dimensional cell migration is regulated by rho/ROCK-dependent matrix reorganization. Biophys. J. 95, (11), 5374 (2008).
  13. Sander, E., Barocas, V., Tranquillo, R. Initial fiber alignment pattern alters extracellular matrix synthesis in fibroblast-populated fibrin gel cruciforms and correlates with predicted tension. Ann. Biomed. Eng. 39, (2), 714-729 (2011).
  14. Barocas, V. H., Tranquillo, R. T. An anisotropic biphasic theory of tissue-equivalent mechanics: the interplay among cell traction, fibrillar network deformation, fibril alignment, and cell contact guidance. J. Biomech. Eng. 119, 137-146 (1997).
  15. Neidert, M. R., Tranquillo, R. T. Tissue-engineered valves with commissural alignment. Tissue Eng. 12, (4), 891-903 (2006).
  16. Robinson PS,, Robinson PS, Planar biaxial behavior of fibrin-based tissue-engineered heart valve leaflets. Tissue Eng. Part A. 15, (10), 2763-2772 (2009).
  17. Grassl, E., Oegema, T., Tranquillo, R. Fibrin as an alternative biopolymer to type I collagen for the fabrication of a media equivalent. J. Biomed. Mater. Res. 60, (4), 607-612 (2002).
  18. Paten, J. A., Zareian, R., Saeidi, N., Melotti, S. A., Ruberti, J. W. Design and performance of an optically accessible, low-volume, mechanobioreactor for long-term study of living constructs. Tissue Eng. Part C Methods. 17, (7), 775-788 (2001).
  19. Ye KY,, Sulli van KE,, Black LD, Encapsulation of cardiomyocytes in a fibrin hydrogel for cardiac tissue engineering. J. Vis. Exp. (55), (2011).
  20. Ahmann, K. A., Weinbaum, J. S., Johnson, S. L., Tranquillo, R. T. Fibrin degradation enhances vascular smooth muscle cell proliferation and matrix deposition in fibrin-based tissue constructs fabricated in vitro. Tissue Eng. Part A. 16, (10), 3261-3270 (2010).
  21. Raghupathy, R., Witzenburg, C., Lake, S. P., Sander, E. A., Barocas, V. H. Identification of regional mechanical anisotropy in soft tissue analogs. J. Biomech. Eng. 133, (2011).
  22. Robinson, P. S., Johnson, S. L., Evans, M. C., Barocas, V. H., Tranquillo, R. T. Functional tissue-engineered valves from cell-remodeled fibrin with commissural alignment of cell-produced collagen. Tissue Eng. Part A. 14, (1), 83-95 (2008).
  23. Gabbiani, G. The myofibroblast in wound healing and fibrocontractive diseases. J. Pathol. 200, (4), 500-503 (2003).
  24. PaszeK, M. J., et al. Tensional homeostasis and the malignant phenotype. Cancer Cell. 8, (3), 241-254 (2005).
  25. Grinnell, F., Rocha L, B., Iucu, C., Rhee, S., Jiang, H. Nested collagen matrices: A new model to study migration of human fibroblast populations in three dimensions. Exp. Cell. Res. 312, (1), 86-94 (2006).
  26. Miron-Mendoza, M., Seemann, J., Grinnell, F. The differential regulation of cell motile activity through matrix stiffness and porosity in three dimensional collagen matrices. Biomaterials. 31, (25), 6425-6435 (2010).
  27. Wakatsuki, T., Kolodney MS,, Zahalak GI,, Elson EL, Cell mechanics studied by a reconstituted model tissue. Biophys. J. 79, (5), 2353-2368 (2000).
  28. De Jesus, A., Aghvami, M., Sander, E. Fibroblast-mediated fiber realignment in fibrin gels. ASME Summer Bioengineering Conference, 2013 Jun 26-29, Sunriver, OR, (2013).
  29. Aghvami, M., Barocas, V. H., Sander, E. A. Multiscale mechanical simulations of cell compacted collagen gels. J. Biomech. Eng. 135, (2013).



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