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JoVE Journal Biology
Disseksjon av
Sigma's Non-specific Protease Activity Assay - Casein as a Substrate
Journal (Biology)
Sigma's Non-specific Protease Activity Assay - Casein as a Substrate
Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA
Journal (Biology)
Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA
 
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Disseksjon av

Article DOI: 10.3791/51118-v • 09:07 min • March 4th, 2014
March 4th, 2014 •
1,2,3,4, 1,2,3,4
1Institut Curie, Centre Universitaire, 2Université Paris Sud, Centre Universitaire, 3CNRS UMR 3347, Centre Universitaire, 4INSERM U1021, Centre Universitaire

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Summary
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Denne protokollen beskriver hvordan å dissekere premigratory kranie neural crest (NC) fra Xenopus laevis neurulas. Disse explants kan bli belagt på fibronectin og dyrket in vitro, eller i podet inn i vertsembryoer. Denne teknikken gjør det mulig å studere mekanismene for NC epitel-til-mesenchyme overgang, migrasjon, og differensiering.

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Developmental Biology Neural crest, Embryo disseksjon pode fibronektin
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