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JoVE Journal Biology
Dissezione dei
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Journal (Biology)
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Journal (Biology)
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Dissezione dei

Article DOI: 10.3791/51118 • 09:07 min
March 4th, 2014 •
1,2,3,4, 1,2,3,4
1Institut Curie, Centre Universitaire, 2Université Paris Sud, Centre Universitaire, 3CNRS UMR 3347, Centre Universitaire, 4INSERM U1021, Centre Universitaire

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March 4th, 2014

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Questo protocollo descrive come sezionare premigratory cresta neurale cranica (NC) da Xenopus laevis neurulas. Tali espianti possono essere placcati in fibronectina e coltivate in vitro, o innestate di nuovo in embrioni di accoglienza. Questa tecnica consente di studiare i meccanismi di NC epitelio a mesenchima transizione, migrazione e differenziazione.

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