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JoVE Journal Biology
Dissekering av
Assaying the Kinase Activity of LRRK2 in vitro
Journal (Biology)
Assaying the Kinase Activity of LRRK2 in vitro
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Journal (Biology)
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Dissekering av

Article DOI: 10.3791/51118-v • 09:07 min • March 4th, 2014
March 4th, 2014 •
1,2,3,4, 1,2,3,4
1Institut Curie, Centre Universitaire, 2Université Paris Sud, Centre Universitaire, 3CNRS UMR 3347, Centre Universitaire, 4INSERM U1021, Centre Universitaire

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Detta protokoll beskriver hur man dissekera premigratory cranial neural crest (NC) från Xenopus laevis neurulas. Dessa explants kan pläterade på fibronektin-och odlas in vitro, eller ympas tillbaka in embryon värd. Denna teknik gör det möjligt att studera mekanismerna för NC epitel-till-mesenkym övergång, migration och differentiering.

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Utvecklingsbiologi Neural crest, Embryo dissektion transplantat fibronektin
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