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Disseksjon av<em> Xenopus laevis</em> Neural Crest for<em> In vitro</em> Eksplantering kultur eller<em> In vivo</em> Transplantasjon
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Dissection of Xenopus laevis Neural Crest for in vitro Explant Culture or in vivo Transplantation

Disseksjon av<em> Xenopus laevis</em> Neural Crest for<em> In vitro</em> Eksplantering kultur eller<em> In vivo</em> Transplantasjon

DOI:
10.3791/51118-v

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09:07 min

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March 04, 2014

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,
1Institut Curie,Centre Universitaire, 2Université Paris Sud,Centre Universitaire, 3CNRS UMR 3347,Centre Universitaire, 4INSERM U1021,Centre Universitaire

Chapters

  • 00:05Title
  • 01:19Dissection of the neural Crest (NC) Explants
  • 03:54Plating Explants on Fibronectin for in vitro Migration Imaging
  • 05:03Grafting Explants into Host Embryos for in vivo Migration Analysis
  • 06:25Results: In vitro and in vivo Behavior of Explanted Cranial NC
  • 08:21Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokollen beskriver hvordan å dissekere premigratory kranie neural crest (NC) fra Xenopus laevis neurulas. Disse explants kan bli belagt på fibronectin og dyrket in vitro, eller i podet inn i vertsembryoer. Denne teknikken gjør det mulig å studere mekanismene for NC epitel-til-mesenchyme overgang, migrasjon, og differensiering.

Tags

Neural CrestXenopus LaevisDissectionExplant CultureIn Vivo TransplantationEpithelium-to-mesenchyme TransitionMigrationDifferentiation

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