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剖析<em>非洲爪蟾</em>神经嵴的<em>在体外</em>外植体培养或<em>在体内</em>移植
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JoVE Journal Biology
Dissection of Xenopus laevis Neural Crest for in vitro Explant Culture or in vivo Transplantation
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剖析<em>非洲爪蟾</em>神经嵴的<em>在体外</em>外植体培养或<em>在体内</em>移植

DOI:
10.3791/51118-v

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09:07 min

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March 04, 2014

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,
1Institut Curie,Centre Universitaire, 2Université Paris Sud,Centre Universitaire, 3CNRS UMR 3347,Centre Universitaire, 4INSERM U1021,Centre Universitaire

Chapters

  • 00:05Title
  • 01:19Dissection of the neural Crest (NC) Explants
  • 03:54Plating Explants on Fibronectin for in vitro Migration Imaging
  • 05:03Grafting Explants into Host Embryos for in vivo Migration Analysis
  • 06:25Results: In vitro and in vivo Behavior of Explanted Cranial NC
  • 08:21Conclusion

Summary

Automatic Translation

Automatic Translation

本协议描述了如何从非洲爪蟾 neurulas解剖premigratory颅神经嵴（NC）。这些外植体可以镀在纤连蛋白和体外培养，或接枝回主机胚胎。这种技术可以让学习数控上皮至间质转变，迁移和分化的机制。

Tags

Keywords: Neural CrestXenopus LaevisDissectionExplant CultureIn Vivo TransplantationEpithelium-to-mesenchyme TransitionMigrationDifferentiation

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