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Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish
JoVE Journal
Biology
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JoVE Journal Biology
Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish
DOI:

07:28 min

February 27, 2014

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Chapters

  • 00:05Title
  • 01:55Preparation of Fluorinated Ethylene Propylene Tubes
  • 03:02Preparation of Zebrafish and Multilayer Mounting
  • 06:26Results: Zebrafish Multi-day Imaging
  • 06:59Conclusion

Summary

Automatic Translation

The development of zebrafish can be followed over days with light sheet microscopy when embryos are embedded in optically clear polymer tubes with low-concentration agarose.

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