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Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
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JoVE Journal Medicine
Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

DOI:
10.3791/51172-v

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09:26 min

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January 22, 2014

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, , ,
1Medical School and Stanley S. Scott Cancer Center,LSU Health Sciences Center, 2Department of Biomedical, Surgery and Dental Sciences,University of Milan

Chapters

  • 00:05Title
  • 01:24Reverse Transcription
  • 03:22Real-time PCR Amplification
  • 05:20Real-time PCR Data Analysis
  • 06:24Inter-plate Data Preparation and Analysis
  • 08:18Results: miRNA Expression Profiles from Cerebrospinal Fluid
  • 09:05Conclusion

Summary

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We describe a protocol of real time PCR to profile microRNAs in the cerebrospinal fluid (CSF). With the exception of RNA extraction protocols, the procedure can be extended to RNA extracted from other body fluids, cultured cells, or tissue specimens.

Tags

Cerebrospinal FluidMicroRNAMiRNAQPCRGene RegulationBiomarkerProfilingExiqonGenEx Professional 5

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