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Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation
JoVE Journal
Biology
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JoVE Journal Biology
Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation
DOI:

14:44 min

March 14, 2014

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Chapters

  • 00:05Title
  • 01:05Mitochondria Purification
  • 06:36RNA Extraction
  • 07:59Preparing RNA for Microarray Analysis
  • 10:04Results: Isolation of Mitochondria-associated mRNA by Subcellular Fractionation
  • 14:13Conclusion

Summary

Automatic Translation

Many mRNAs encoding mitochondrial proteins are associated with the mitochondria outer membrane. We describe a subcellular fractionation procedure aimed at isolation of yeast mitochondria with its associated mRNAs and ribosomes. This protocol can be applied to cells grown under diverse conditions in order to reveal mechanisms of mRNA localization and localized translation near the mitochondria.

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