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JoVE Journal Bioengineering
A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
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A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Article DOI: 10.3791/51797-v • 14:53 min • September 10th, 2014
September 10th, 2014 •
1, 1, 2, 2, 1
1MEMS Sensors and Actuators Laboratory (MSAL), Department of Electrical and Computer Engineering, Institute for Systems Research, University of Maryland, 2Institute for Bioscience and Biotechnology Research, Fischell Department of Bioengineering, University of Maryland

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Summary
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We present a microfluidic-based electrochemical biochip for DNA hybridization detection. Following ssDNA probe functionalization, the specificity, sensitivity, and detection limit are studied with complementary and non-complementary ssDNA targets. Results illustrate the influence of the DNA hybridization events on the electrochemical system, with a detection limit of 3.8 nM.

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Microfluidic-based Electrochemical Biochip Label-free DNA Hybridization Analysis Miniaturization Analytical Benchtop Procedures Micro-scale Reaction Time Cost Integration Pre-processing Steps DNA Hybridization Events Real-time Assessment Biomarkers Point-of-care Diseases Device Footprint Physical Phenomena Fabrication Precision Operation Reliability Reproducible Manner Overall Performance Protocols Methods Microfluidic Chip Polydimethylsiloxane (PDMS) Electrochemical Microchip Electrochemical Impedance Spectroscopy (EIS) Charge Transfer Diffusional Resist
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