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Creating Anatomically Accurate and Reproducible Intracranial Xenografts of Human Brain Tumors
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Creating Anatomically Accurate and Reproducible Intracranial Xenografts of Human Brain Tumors

Creating Anatomically Accurate and Reproducible Intracranial Xenografts of Human Brain Tumors

DOI:
10.3791/52017-v

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11:14 min

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September 24, 2014

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1Department of Pediatrics,University of Colorado School of Medicine

Chapters

  • 00:05Title
  • 01:32Equipment Preparation
  • 02:32Cell Preparation
  • 03:19Surgical Preparation
  • 04:10Intracranial Injection
  • 08:10Recovery & Tumor Development
  • 08:55Results: Tumor Growth After Intracranial Injection
  • 10:31Conclusion

Summary

Automatic Translation

Automatic Translation

The brain is a unique site with qualities that are not well represented by in vitro or ectopic analyses. Orthotopic mouse models with reproducible location and growth characteristics can be reliably created with intracranial injections using a stereotaxic fixation instrument and a low pressure syringe pump.

Tags

Orthotopic Tumor ModelsBrain TumorsIntracranial XenograftsHuman Brain TumorsIn Vivo ModelsTumor Cell EngraftmentSurgical TechniqueReproducibilityTumor GrowthCustomization

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