Journal
/
/
Glutamate and Hypoxia as a Stress Model for the Isolated Perfused Vertebrate Retina
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Glutamate and Hypoxia as a Stress Model for the Isolated Perfused Vertebrate Retina
DOI:

07:48 min

March 22, 2015

, , , , , , , ,

Chapters

  • 00:05Title
  • 02:04Preparation of Bovine Eyes
  • 03:15Recording the Electroretinogram
  • 04:23Data Analysis
  • 05:23Results: Effects of Hypoxia or Glutamate on Retinal a- and b-wave Amplitudes
  • 07:10Conclusion

Summary

Automatic Translation

With this study, we introduce a standardized stress model for the isolated superfused bovine retina for future preclinical therapeutic testing. The effect of either hypoxia (pure N2) or glutamate stress (250 µM glutamate) on retinal function represented by a- and b-wave amplitudes was evaluated.

Related Videos

Read Article